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Interrogating the Function of Bicistronic Translational Control Elements to Improve Consistency of Gene Expression.

Jansen Z., Reilly S.R., Lieber-Kotz M., Li A.Z., Wei Q., Kulhanek D.L., Gilmour A.R., Thyer R.

ACS Synth Biol. 2023 Jun 16;12(6):1608-1615. doi: 10.1021/acssynbio.3c00093.

PMID: 37253269

Integrated Top-Down and Bottom-Up Mass Spectrometry for Characterization of Diselenide Bridging Patterns of Synthetic Selenoproteins.

Watts E., Thyer R., Ellington A.D., Brodbelt J.S.

Anal Chem. 2022 Aug 16;94(32):11175-11184. doi: 10.1021/acs.analchem.2c01433.

PMID: 35930618

Using fungible biosensors to evolve improved alkaloid biosyntheses.

d'Oelsnitz S., Kim W., Burkholder N.T., Javanmardi K., Thyer R., Zhang Y., Alper H.S., Ellington A.D.

Nat Chem Biol. 2022 Sep;18(9):981-989. doi: 10.1038/s41589-022-01072-w.

PMID: 35799063

DLPacker: Deep Learning for Prediction of Amino Acid Side Chain Conformations in Proteins.

Misiura, M., Shroff, R., Thyer, R., and Kolomeisky, A.B.

Proteins. 2022 Jun;90(6):1278-1290. doi: 10.1002/prot.26311.

PMID: 35122328

Directed evolution of an improved aminoacyl-tRNA synthetase for incorporation of L-3,4-dihydroxyphenylalanine (L-DOPA).

Thyer, R., d'Oelsnitz, S., Blevins, M.S., Klein, D.R., Brodbelt, J.S., and Ellington, A.D.

Angew Chem Int Ed Engl . 2021 Apr 19. doi: 10.1002/anie.202100579

PMID: 33871147

Discovery of novel gain-of-function mutations guided by structure-based deep learning.
*Shroff, R., *Cole, A.W., Morrow, B.R., Diaz, D.J., Donnell, I., Annapareddy, A., Gollihar, J., Ellington, A.D. and Thyer, R. ACS Synth. Biol. 2020 Nov 20; 9(11):2927-2935. doi: 10.1021/acssynbio.0c00345

PMID: 33064458

The role of tRNA in establishing new genetic codes.
Thyer, R. and Ellington, A.D.

Biochemistry. 2019 Mar 19; 58(11):1460-1463. doi: 10.1021/acs.biochem.8b00834

PMID: 30387992


Custom selenoprotein production enabled by laboratory evolution of recoded bacterial strains.
Thyer, R., Shroff, R., Klein, D.R., d'Oelsnitz, S., Cotham, V.C., Byrom, M., Brodbelt, J.S. and Ellington, A.D.
Nat. Biotech. 2018 Jun 8; (36):624–631. doi:10.1038/nbt.4154

PMID: 29863724


Characterization of trimethoprim resistant E. coli dihydrofolate reductase mutants by mass spectrometry and inhibition by propargyl-linked antifolates.

Cammarata, M.B., Thyer, R., Lombardo, M., Anderson, A., Wright, D., Ellington, A.D. and Brodbelt, J.S.
Chem. Sci. 2017 Mar 24; 8(5):4062-4072. doi:10.1039/c6Sc05235e

PMID: 29967675


Addicting diverse bacteria to a noncanonical amino acid.
Tack, D.S., Ellefson, J.W., Thyer, R., Wang, B., Gollihar, J., Forster, M.T., Ellington, A.D.
Nat. Chem. Biol. 2016 Mar; 12(3):138-40. doi: 10.1038/nchembio.2002


Structural characterization of dihydrofolate reductase complexes by top-down ultraviolet photodissociation mass spectrometry.
Cammarata, M.B., Thyer, R., Rosenberg, J., Ellington, A.D., Brodbelt, J.S.
J. Am. Chem. Soc. 2015 Jul 22; 137(28):9128-35. doi: 10.1021/jacs.5b04628.


Evolving tRNA(Sec) for efficient canonical incorporation of selenocysteine.
Thyer, R., Robotham, S.A., Brodbelt, J.S., Ellington, A.D.
J. Am. Chem. Soc. 2015 Jan 14; 137(1):46-9. doi: 10.1021/ja510695g.


Synthetic biology: New letters for life's alphabet.
Thyer, R., Ellefson, J.W.
Nature. 2014 May 15; 509(7500):291-2. doi: 10.1038/nature13335.


Engineered rRNA enhances the efficiency of selenocysteine incorporation during translation.
Thyer, R., Filipovska, A., Rackham, O.
J. Am. Chem. Soc. 2013 Jan 9; 135(1):2-5. doi: 10.1021/ja3069177.


Substrate and inhibitor specificities differ between human cytosolic and mitochondrial thioredoxin reductases: Implications for development of specific inhibitors.
Rackham, O., Shearwood, A.M., Thyer, R., McNamara, E., Davies, S.M., Callus, B.A., Miranda-Vizuete, A., Berners-Price, S.J., Cheng, Q., Arnér, E.S., Filipovska, A.
Free Radic. Biol. Med. 2011 Mar 15; 50(6):689-99. doi: 10.1016/j.freeradbiomed.2010.12.015.

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