PUBLICATIONS
Jansen Z., Reilly S.R., Lieber-Kotz M., Li A.Z., Wei Q., Kulhanek D.L., Gilmour A.R., Thyer R.
ACS Synth Biol. 2023 Jun 16;12(6):1608-1615. doi: 10.1021/acssynbio.3c00093.
PMID: 37253269
Integrated Top-Down and Bottom-Up Mass Spectrometry for Characterization of Diselenide Bridging Patterns of Synthetic Selenoproteins.
Watts E., Thyer R., Ellington A.D., Brodbelt J.S.
Anal Chem. 2022 Aug 16;94(32):11175-11184. doi: 10.1021/acs.analchem.2c01433.
PMID: 35930618
Using fungible biosensors to evolve improved alkaloid biosyntheses.
d'Oelsnitz S., Kim W., Burkholder N.T., Javanmardi K., Thyer R., Zhang Y., Alper H.S., Ellington A.D.
Nat Chem Biol. 2022 Sep;18(9):981-989. doi: 10.1038/s41589-022-01072-w.
PMID: 35799063
DLPacker: Deep Learning for Prediction of Amino Acid Side Chain Conformations in Proteins.
Misiura, M., Shroff, R., Thyer, R., and Kolomeisky, A.B.
Proteins. 2022 Jun;90(6):1278-1290. doi: 10.1002/prot.26311.
PMID: 35122328
Directed evolution of an improved aminoacyl-tRNA synthetase for incorporation of L-3,4-dihydroxyphenylalanine (L-DOPA).
Thyer, R., d'Oelsnitz, S., Blevins, M.S., Klein, D.R., Brodbelt, J.S., and Ellington, A.D.
Angew Chem Int Ed Engl . 2021 Apr 19. doi: 10.1002/anie.202100579
PMID: 33871147
Discovery of novel gain-of-function mutations guided by structure-based deep learning.
*Shroff, R., *Cole, A.W., Morrow, B.R., Diaz, D.J., Donnell, I., Annapareddy, A., Gollihar, J., Ellington, A.D. and Thyer, R. ACS Synth. Biol. 2020 Nov 20; 9(11):2927-2935. doi: 10.1021/acssynbio.0c00345
PMID: 33064458
The role of tRNA in establishing new genetic codes.
Thyer, R. and Ellington, A.D.
Biochemistry. 2019 Mar 19; 58(11):1460-1463. doi: 10.1021/acs.biochem.8b00834
PMID: 30387992
Custom selenoprotein production enabled by laboratory evolution of recoded bacterial strains.
Thyer, R., Shroff, R., Klein, D.R., d'Oelsnitz, S., Cotham, V.C., Byrom, M., Brodbelt, J.S. and Ellington, A.D.
Nat. Biotech. 2018 Jun 8; (36):624–631. doi:10.1038/nbt.4154
PMID: 29863724
Characterization of trimethoprim resistant E. coli dihydrofolate reductase mutants by mass spectrometry and inhibition by propargyl-linked antifolates.
Cammarata, M.B., Thyer, R., Lombardo, M., Anderson, A., Wright, D., Ellington, A.D. and Brodbelt, J.S.
Chem. Sci. 2017 Mar 24; 8(5):4062-4072. doi:10.1039/c6Sc05235e
PMID: 29967675
Addicting diverse bacteria to a noncanonical amino acid.
Tack, D.S., Ellefson, J.W., Thyer, R., Wang, B., Gollihar, J., Forster, M.T., Ellington, A.D.
Nat. Chem. Biol. 2016 Mar; 12(3):138-40. doi: 10.1038/nchembio.2002
PMID:26780407
Structural characterization of dihydrofolate reductase complexes by top-down ultraviolet photodissociation mass spectrometry.
Cammarata, M.B., Thyer, R., Rosenberg, J., Ellington, A.D., Brodbelt, J.S.
J. Am. Chem. Soc. 2015 Jul 22; 137(28):9128-35. doi: 10.1021/jacs.5b04628.
PMID:26125523
Evolving tRNA(Sec) for efficient canonical incorporation of selenocysteine.
Thyer, R., Robotham, S.A., Brodbelt, J.S., Ellington, A.D.
J. Am. Chem. Soc. 2015 Jan 14; 137(1):46-9. doi: 10.1021/ja510695g.
PMID:25521771
Synthetic biology: New letters for life's alphabet.
Thyer, R., Ellefson, J.W.
Nature. 2014 May 15; 509(7500):291-2. doi: 10.1038/nature13335.
PMID:24805244
Engineered rRNA enhances the efficiency of selenocysteine incorporation during translation.
Thyer, R., Filipovska, A., Rackham, O.
J. Am. Chem. Soc. 2013 Jan 9; 135(1):2-5. doi: 10.1021/ja3069177.
PMID:23256865
Substrate and inhibitor specificities differ between human cytosolic and mitochondrial thioredoxin reductases: Implications for development of specific inhibitors.
Rackham, O., Shearwood, A.M., Thyer, R., McNamara, E., Davies, S.M., Callus, B.A., Miranda-Vizuete, A., Berners-Price, S.J., Cheng, Q., Arnér, E.S., Filipovska, A.
Free Radic. Biol. Med. 2011 Mar 15; 50(6):689-99. doi: 10.1016/j.freeradbiomed.2010.12.015.
PMID:21172426