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Testing our fluorescence imager

Updated: Oct 16, 2020

Our custom fluorescence imager is a work in progress.

Imaging cell pellets for near-IR fluorescence.

We frequently need to perform fluorescent imaging using excitation wavelengths other than those offered by RGB LEDs. Omega Lum series gel imagers (Gel Company) are good options for research groups needing to use non-standard excitation and emission wavelengths. These models have a six position filter wheel with standard 58 mm photographic threads and ample space within the chamber to install additional excitation modules. Using a 595 nm amber LED and an 850 nm optical bandpass filter (Midwest Optical Systems) we successfully imaged cells expressing near-IR fluorescent proteins. I'll provide another update once we get a full suite of LEDs installed.

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